The St. Charles Streetcar Line is a historic streetcar line in New Orleans, Louisiana. Running since 1835, it is the oldest continuously operating streetcar line in the world. It is operated by the New Orleans Regional Transit Authority (RTA). Officially the St. Charles Streetcar line is designated as Route 12, and it runs along its namesake, St. Charles Avenue. It is the busiest route in the RTA system as it is heavily used by local commuters and tourists. On most RTA maps and publications, it is denoted in green, which is also the color of the streetcars on this line.

The Canal Streetcar Line is a streetcar line in New Orleans, Louisiana, operated by the New Orleans Regional Transit Authority (RTA). The line originally operated from 1861 until its closure in 1964. After a 40-year hiatus, it was rebuilt between 2000 and 2004, with streetcar service resuming on April 18, 2004.

The Rampart–Loyola Streetcar Line is a historic streetcar line in New Orleans, Louisiana. It is operated by the New Orleans Regional Transit Authority (RTA). It is the newest streetcar line in the system, as it opened in its original form on January 28, 2013, with the total length of the line being 2.4 mi (3.9 km). The line is officially designated Route 46 and is denoted with a purple color on most RTA publications.

The Riverfront Streetcar Line is a streetcar route in New Orleans, Louisiana, operated by the New Orleans Regional Transit Authority  (RTA). It opened on August 14, 1988, becoming the first new streetcar line in the city in 62 years. The route runs for 2 miles (3.2 km. along the east bank of the Mississippi River, between Esplanade Avenue in the French Quarter and Julia Street near the New Orleans Convention Center.