The tarantula hawk wasp is their top predator

The tarantula hawk wasps (also called the parasitic pepsis wasp) hunt tarantulas. The female wasp stings the tarantula to paralyze it. Then, it carries the tarantula's body back to the burrow and lays eggs on the spider's body. When the egg hatches, the wasp larvae will have a ready food source in the paralyzed spider, which they feed on as they grow.